大肠杆菌 E.Coli 蛋白表达
蛋白表达纯化与制备平台具有全套原核蛋白表达与纯化仪器系统,提供从 PCR 克隆载体构建、原核培养蛋白表达到菌体破碎蛋白纯化的全套服务。
原核表达、纯化一般流程
从载体构建到纯化鉴定的完整链路,各环节均在平台内完成,仪器与试剂耗材配套齐备。
蛋白表达纯化与制备平台具有全套原核蛋白表达与纯化仪器系统,从PCR克隆载体构建,到E.coli原核培养蛋白表达,再到菌体破碎蛋白纯化,我们能够提供全套仪器使用服务和试剂耗材服务。
- 01 PCR 克隆与载体构建
- 02 E.coli 原核培养与蛋白表达
- 03 菌体破碎与蛋白纯化
- 04 纯度鉴定与结果交付
平台仪器配置
覆盖克隆、培养、破碎、离心、纯化与鉴定各环节的主要设备。













平台质粒与感受态细胞
平台常备多种表达质粒与配套感受态细胞,可按项目需求选择。
| 平台质粒 | 感受态细胞 |
|---|---|
| pET29b | BL21(anti-phage) |
| pET15D | DH5a |
| pET21b | Top10 |
| pMBP | DH10Bac |
| pPGH | Rosseta |
| pBB75 | Origammi |
| pQlink | C43 |
| pQlink-His | — |
| pQlink-GST | — |
技术交流
部分客户署名公司发表文章:
- 01IF 40.8
SARS-CoV-2 envelope protein impairs airway epithelial barrier function and exacerbates airway inflammation via increased intracellular Cl− concentration.
Signal Transduction and Targeted Therapy (2024) 9:74
- 02IF 14.7
Malaria oocysts require circumsporozoite protein to evade mosquito immunity.
NATURE COMMUNICATIONS (2022) 13:3208
- 03IF 12.085
Identification and functional characterization of a novel BEL1-LIKE homeobox transcription factor GmBLH4 in soybean.
https://doi.org/10.1007/s11240-018-1419-4
- 04IF 10
Nanopore efficiently identifies hepatitis D virus antigens in vitro assay.
Materials Today Physics 46 (2024) 101479
- 05IF 9.4
Integration of genome wide association studies and co-expression networks reveal roles of PtoWRKY 42-PtoUGT76C1-1 in trans-zeatin metabolism and cytokinin sensitivity in poplar.
New Phytologist (2021) 231: 1462–1477, doi: 10.1111/nph.1746
- 06IF 9.2
Targeting HLA-F suppresses the proliferation of glioma cells via a reduction in hexokinase 2-dependent glycolysis.
International Journal of Biological Sciences. Published: 2021.03.25, doi:10.7150/ijbs.56357
- 07IF 7.3
Discovery of Clinical Candidate (5-(3-(4-Chlorophenoxy)prop-1-yn-1-yl)-3-hydroxypicolinoyl)glycine, an Orally Bioavailable Prolyl Hydroxylase Inhibitor for the Treatment of Anemia.
2020, 63, 17, 10045–10060
- 08IF 6.2
Adenosine Monophosphate-Activated Protein Kinase (AMPK) Phosphorylation Is Required for 20-Hydroxyecdysone Regulates Ecdysis in Apolygus lucorum.
Int J Mol Sci. 2023 May 11;24(10):8587, doi: 10.3390/ijms24108587
- 09IF 7.1
The major-effect quantitative trait locus CsARN6.1 encodes an AAA ATPase domain-containing protein that is associated with waterlogging stress tolerance by promoting adventitious root formation.
2018 Mar;93(5):917-930, doi: 10.1111/tpj.13819. Epub 2018 Feb 2
案例展示
下列结果为平台实际项目中的代表性展示,涵盖纯度、条件优化与表达鉴定等环节。
表达条件优化
蛋白表达鉴定
蛋白表达纯化
相关服务
如需其他表达体系或下游表征支持,可参考以下服务。
